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KMID : 0361020080510010028
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Korean Journal of Otolaryngology - Head and Neck Surgery
2008 Volume.51 No. 1 p.28 ~ p.32
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Isolation and Culture of Adult Neural Stem Cells from Guinea Pig Tympanic Membrane
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Park Kyoung-Ho
Yeo Sang-Won
Chang Ki-Hong
Park Shi-Nae
Lee Byung-Hoon
Bae Seong-Cheon
Sung Yong-Hae
Bae Min-Yung
Kim Jun-Gyu
Lee Chang-Hoon
Kim Boo-Young
Baek Jee-Hye
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Abstract
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Background & Objectives: Tympanic membrane perforation is an important clinical problem found in various populations of the world. In large number of cases, acute traumatic perforations heal spontaneously, and in the healing process, stem cells appear to play an important role. However, no studies have been reported regarding somatic stem cells in the tympanic membrane. Herein, we tried to show that guinea pig’s tympanic membrane contains cells that display the characteristic features of stem cells.
Materials & Method: The tympanic membrane was obtained from the guinea pig. The cells were cultured in a medium with epidermal growth factor (EGF) and fibroblast growth factor (FGF). Proliferating cells were checked with stem cell markers, bromodeoxyuridine (BrdU) and nestin. Differentiated cells from stem cells are checked with betaIII tubulin and S-100.
Results: We observed that some of the cultured cells from the tympanic membrane were stained with both stem cell markers, BrdU and nestin. And we observed that these cells differentiated into neuron and gilal cells, which expressed betaIII tubulin and S-100, respectively.
Conclusions: These results suggest that the tympanic membrane of guinea pigs may have neural stem cells. Further study is needed for finding the origin of stem cells.
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KEYWORD
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Tympanic membrane, Adult stem cell
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